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Table 1 Primers sequence and PCR conditions for molecular characterization of microscopy-positive and serology-positive samples

From: Determining intestinal parasitic infections (IPIs) in inmates from Kajang Prison, Selangor, Malaysia for improved prison management

Species

PCR reaction

Primers set

Primers sequence (5’-3’)

PCR conditions

Total cycles (2–4)f

Product size

Reference

    

1a

2b

3c

4d

5e

   

Blastocystis sp.

 

Blast 505–532

F-GGA GGT AGT GAC AAT AAA TC

95 °C, 4 min

95 °C, 30 s

54 °C, 30 s

72 °C, 30 s

72 °C, 5 min

35

500 bp

Böhm-Gloning et al., 1997

 

Blast 998–1017

R TGC TTT CGC ACT TGT TCA TC

Santin et al., 2011

S. stercoralis

Primary

SS–FO

F-ATC CTT CCA ATC GCT GTT GT

94 °C, 5 min

94 °C, 45 s

58 °C, 60 s

72 °C, 1 min

72 °C, 5 min

35

 

Nilforoushan et al., 2007

 

SS–RO

R-TTT CGT GAT GGG CTA ATT CC

Secondary

SS–FI

F-GTA ACA AGG TTT TCG TAG GTG A

94 °C, 2 min

94 °C, 45 s

60 °C, 45 s

72 °C, 1 min

72 °C, 5 min

30

680 bp

 
 

SS–RI

R-ATT TAG TTT CTT TTC CTC CGC TT

 

Entamoeba

Primary

E-1

F-TAA GAT GCA CGA GAG CGA AA

96 °C, 2 min

92 °C, 1 min

56 °C, 1 min

72 °C, 1 min 30 s

72 °C, 7 min

30

 

Khairnar & Parija, 2007

  

E-2

R-GTA CAA AGG GCA GGG ACG TA

        

E. histolytica

Secondary

EH-1

F-AAG CAT TGT TTC TAG ATC TGA G

96 °C, 2 min

92 °C, 1 min

48 °C, 1 min

72 °C, 1 min 30 s

72 °C, 7 min

 

439 bp

 
  

EH-2

R-AAG AGG TCT AAC CGA AAT TAG

        

E.dispar

 

ED-1

F-TCT AAT TTC GAT TAG AAC TCT

      

174 bp

 
  

ED-2

R-TCC CTA CCTATT AGA CAT AGC

        

E. moshkovskii

 

Mos-1

F-GAA ACC AAG AGT TTC ACA AC

      

553 bp

 
  

Mos-2

R-CAA TAT AAG GCT TGG ATG AT

        
       

Reference

        

5

   

C. parvum

Primary

N-DIAGF2

F-CAA TTG GAG GGC AAG TCT GGT GCC AGC

95 °C, 5 min

94 °C, 30 sec

68 °C, 1 min

72 °C, 30 sec

72 °C, 10 min

35

655 to 667 bp

Nichols et al., 2003

  

N-DIAGR2

R-CCT TCC TAT GTC TGG ACC TGG TGA GT

        
 

Secondary

CPB-DIAGF

F-AAG CTC GTA GTA GTT GGA TTC TG

95 °C, 5 min

94 °C, 30 sec

60 °C, 1 min

72 °C, 30 sec

72 °C, 10 min

 

435 bp

Johnson et al., 1995

  

CPB-DIAGR

R-TAA GGT GCT GAA GGA GTA AGG

        
  1. aInitial denaturation
  2. bDenaturation
  3. cAnnealing
  4. dExtension
  5. eFinal extension
  6. fTotal cycles only applicable for PCR conditions 2–4