Fig. 3

Detection of wildtype and mutated VLPs by ELISA with epitope-specific antibodies. Equal amounts of VLPs were lysed, directly coated on ELISA plates and detected either with epitope-specific antibodies (a: AG3.0 for position 16; c: YDHIV1pg24 for position 170) or pooled HIV+ plasma to control for the presence of antigen (b and d). S/Co ratios were calculated in relation to background signals of the respective antibodies on uncoated wells. Results are averages of three experiments. Lines indicate S/Co =1, above which samples were scored as detectable