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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: Impact of naturally occurring amino acid variations on the detection of HIV-1 p24 in diagnostic antigen tests

Fig. 3

Detection of wildtype and mutated VLPs by ELISA with epitope-specific antibodies. Equal amounts of VLPs were lysed, directly coated on ELISA plates and detected either with epitope-specific antibodies (a: AG3.0 for position 16; c: YDHIV1pg24 for position 170) or pooled HIV+ plasma to control for the presence of antigen (b and d). S/Co ratios were calculated in relation to background signals of the respective antibodies on uncoated wells. Results are averages of three experiments. Lines indicate S/Co =1, above which samples were scored as detectable

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