Fig. 2

Detection of wildtype and mutated VLPs by western blot with epitope-specific antibodies. Position 16 and 170 wildtype and mutated VLPs were detected with the epitope-specific antibody AG3.0 (a) and YDHIV1gp24 (b), respectively. In parallel, the same sample preparations were loaded on a second gel and p24 was detected with pooled HIV+ plasma on the membranes to control for the presence antigen (a and b lower panels)