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Table 3 Re-identification using multigene sequence-based typing, distribution of hsp65, ITS sequevar analysis, and insertion elements

From: Molecular analysis of clinical isolates previously diagnosed as Mycobacterium intracellulare reveals incidental findings of “Mycobacterium indicus pranii” genotypes in human lung infection

 

M. intracellulare (MI)

“M. indicus pranii” (MIP)

Comparison between MI and MIP

Identification and diagnosis by

   

 Non-sequencing method (PRA)

77

0

Identical

 Multigene sequence-based typing

74

3

Different

  16S rRNA

  

Identical

  ITS

  

Identical

  rpoB

  

Different

  hsp65

  

Different

Molecular characterization

   

 Distribution of hsp65 sequevar

  

Differenta

  Code 10

9

0

  Code 11

13

0

  Code 13

48

0

  Code 14

2

0

  Code N4

1

0

  Code N5

1

0

  Code N6

0

3

 ITS sequevar

Min-A

Min-A

Identical

 Insertion elementsb

  

Identical

  IS900

-

-

  IS901

-

-

  IS1311

−/+c

-

  DT1

+

+

  1. PRA, PCR restriction fragment length polymorphism analysis, ITS internal transcribed spacer
  2. aTwo species were not distinguished by previously published hsp65 code, but code N6 identified in this study was different between the two species
  3. bPCR results of insertion element are indicated as positive(+) or negative(−)
  4. cFour isolates identified as M. intracellulare were positive for IS1311
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BMC Infectious Diseases

ISSN: 1471-2334

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