Fig. 1

The intermediate precision of the Kalon HSV-2 IgG ELISA (N = 596). These data are based on the performance of Kalon by Technologist-Jr. and Technologist-Sr. at the HPTN Laboratory Center in the USA. a Shows box plots that represent the range, interquartile range, and median Kalon index values for all samples tested by both operators. b Shows the correlation and variability in Kalon index values between operators. c Presents the agreement of categorical results between operators as determined by the manufacturer’s index cut-offs (<0.9, negative; 0.9–1.1, indeterminate; >1.1 positive)