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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: A single-nucleotide-polymorphism real-time PCR assay for genotyping of Mycobacterium tuberculosis complex in peri-urban Kampala

Fig. 2

Comparing RD 750 LSP-PCR and LRPS typing: Samples (N = 33) were analyzed by primers specific for RD 750 (lane M = DNA marker, 1 = H37Rv, 2 = negative control (no DNA added), lane 3–36 = test samples), a band of 0.75 kb identifies MTB Lineage 3, while that of 1.3 kb identifies other MTBC other than MTB Lineage 3 (See Panel a). For Panel b specific primers and probes (Rv0129cc primer/probe set) containing Lineage-3 specific SNP were used, the top panel shows the amplification (see also Additional file 5: Figure S2) of the target region while the bottom panel shows the derived melting temperature. A peak of 57 °C shows MTB lineage 3 while that of 67 °C shows the wild type (non-lineage 3)

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