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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: A single-nucleotide-polymorphism real-time PCR assay for genotyping of Mycobacterium tuberculosis complex in peri-urban Kampala

Fig. 1

Comparing RD 724 LSP-PCR and LRPS typing: Samples (N = 70) were analyzed by primers specific for RD 724 (lane M = DNA marker, 1 = MTB Uganda genotype, 2 = H37Rv, 3 = negative control (no DNA added), lane 4–72 = test samples) a band of 1.5 kb identifies MTB Uganda family, while that of 1.3 kb identifies other MTBC other than Uganda family (Panel a). For Panel b specific primers/probes (Rv004c primer/probe set) containing lineage specific SNP were used, the top panel shows the amplification (see also Additional file 5: Figure S2a) of the target region while the bottom panel shows the derived melting temperature. A peak of 62 °C shows the Uganda family while that of 68 °C shows the wild type (non-Uganda family)

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BMC Infectious Diseases

ISSN: 1471-2334

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