Skip to main content

Table 3 Measurement of melting temperatures of M. abscessus group from sputa samples by hsp65 PNA real-time PCR

From: Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi-probe- real-time PCR method into sputa samples

 

Channels

Species

FAM

Hex

Texas Red

M. abscessus

 No. of identification

18

18

18

 Average T m (°C)b

50.0 ± 0.85

59.7 ± 0.37

47.1 ± 0.45

 Range of T m (°C)

49.052.3

59.0–60.5

46.3–47.7

 No. of failed detection

0

0

3

M. massiliense Type I

 No. of identification

5

5

5

 Average T m (°C)

61.8 ± 0.37

60.6 ± 0.56

46.9 ± 0.28

 Range of T m (°C)

61.462.1

59.9–61.0

46.5–47.2

 No. of failed detection

0

0

0

M. massiliense Type II-1

 No. of identification

4

4

4

 Average T m (°C)

61.9 ± 0.08

69.1 ± 0.49

52.7 ± 0.89

 Range of T m (°C)

62.063.3

68.471.3

51.553.4

 No. of failed detection

0

0

0

M. massiliense Type II-2

 No. of identification

2

2

2

 Average T m (°C)

62.0 ± 0.07

69.0 ± 0.42

46.6 ± 0.21

 Range of T m (°C)

61.962.0

68.769.3

46.446.7

 No. of failed detection

0

0

0

  1. amean ± SD
  2. bT m , melting temperature; boldface, species specific T m ; −, no significant T m