Skip to main content

Table 3 Measurement of melting temperatures of M. abscessus group from sputa samples by hsp65 PNA real-time PCR

From: Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi-probe- real-time PCR method into sputa samples

  Channels
Species FAM Hex Texas Red
M. abscessus
 No. of identification 18 18 18
 Average T m (°C)b 50.0 ± 0.85 59.7 ± 0.37 47.1 ± 0.45
 Range of T m (°C) 49.052.3 59.0–60.5 46.3–47.7
 No. of failed detection 0 0 3
M. massiliense Type I
 No. of identification 5 5 5
 Average T m (°C) 61.8 ± 0.37 60.6 ± 0.56 46.9 ± 0.28
 Range of T m (°C) 61.462.1 59.9–61.0 46.5–47.2
 No. of failed detection 0 0 0
M. massiliense Type II-1
 No. of identification 4 4 4
 Average T m (°C) 61.9 ± 0.08 69.1 ± 0.49 52.7 ± 0.89
 Range of T m (°C) 62.063.3 68.471.3 51.553.4
 No. of failed detection 0 0 0
M. massiliense Type II-2
 No. of identification 2 2 2
 Average T m (°C) 62.0 ± 0.07 69.0 ± 0.42 46.6 ± 0.21
 Range of T m (°C) 61.962.0 68.769.3 46.446.7
 No. of failed detection 0 0 0
  1. amean ± SD
  2. bT m , melting temperature; boldface, species specific T m ; −, no significant T m