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Table 2 Comparison of identification results between sputum hsp65 PNA real-time PCR and sputum HMPRT-PCR based on same culture-based rpoB PRA

From: Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi-probe- real-time PCR method into sputa samples

  rpoB PRA HMPRT-PCR hsp65 PNA real-time PCR
Sensitivity    
M. abscessus 30 14 18
M. massiliense NA 7 11
  M. massiliense Type I NA NA 5
  M. massiliense Type II-1 NA NA 4
  M. massiliense Type II-2 NA NA 2
Total No. of identification   21 (70 %) 29 (97 %)
No. of misidentification   0 0
Specificity    
 Non-M. abscessus group 30 28 0
  M. abscessus group identified   0 (100 %) 0 (100 %)
No. of misidentification   0 NA
  1. NA, not available