Table 2 Comparison of identification results between sputum hsp65 PNA real-time PCR and sputum HMPRT-PCR based on same culture-based rpoB PRA
rpoB PRA | HMPRT-PCR | hsp65 PNA real-time PCR | |
|---|---|---|---|
Sensitivity | |||
M. abscessus | 30 | 14 | 18 |
M. massiliense | NA | 7 | 11 |
M. massiliense Type I | NA | NA | 5 |
M. massiliense Type II-1 | NA | NA | 4 |
M. massiliense Type II-2 | NA | NA | 2 |
Total No. of identification | 21 (70 %) | 29 (97 %) | |
No. of misidentification | 0 | 0 | |
Specificity | |||
Non-M. abscessus group | 30 | 28 | 0 |
M. abscessus group identified | 0 (100 %) | 0 (100 %) | |
No. of misidentification | 0 | NA |