Fig. 6
Senescence phenotypes of HBMECs and astrocytes are induced by HIV-1 related comorbidity factors and blocked by MLA. (a) SA-βgal images of HBMEC were obtained with the treatment of: (a) mock, (b) 1 μg gp41-I90 for 24 h, (c) Cn cells (104 cells) in a chamber slide for 24 h. Blue spots in (b) & (c) indicate senescence cells. For SAHF images, HBMEC was treated with 1 μg gp41-I90 for 24 h, and stained with an antibody against dimethyl-histone H3 (Lys9). Immunofluorescence microscopic images detect (e) nuclear DNA (DAPI, blue), (f) heterochromatin foci =(SAHF, red), and (g) the overlaid image. Bar = 25 μm. (b) a: Control (HBMECs), b: gp41-I90 (1 μg/ml), c: METH (50 nM), d: gp41-I90 (1 μg/ml) + METH (50 nM), e: MLA+ gp41-I90 (1 μg/ml), f: MLA+ METH (50 nM). (c) a: Control (astrocytes), b: gp41-I90 (1 μg/ml), c: METH (50 nM), d: gp41-I90 (1 μg/ml) + METH (50 nM), e: MLA+ gp41-I90 (1 μg/ml), f: MLA+ METH (50 nM)