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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: Alpha7 nicotinic acetylcholine receptor is required for blood-brain barrier injury-related CNS disorders caused by Cryptococcus neoformans and HIV-1 associated comorbidity factors

Fig. 3

Effects of A7R KO on BBB disorders induced by gp41-I90 and METH. cBMECs were isolated from WT and A7R KO mice treated with gp41-I90 (GP41) or METH as described in our recent publications [9, 49]. Cells without treatment were used as a control. Triple staining (TS) was done by DAPI (blue)/antibodies against CD146 (FITC/green) and S100B (for brain) (rhodamine/red). a: CECs (CD146+/DAPI+), and b: cBMECs (CD146+/S100B+/DAPI+). Cells were counted with six random fields. (*P <0.05; **P < 0.01; ***P < 0.001)

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