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Table 1 Reactivity of purified MAb.F fractions with crude antigens from S. haematobium parasite stages and infected human urine

From: Immunolocalization of the 29 kDa Schistosoma haematobium species-specific antigen: a potential diagnostic marker for urinary schistosomiasis

Antibody fraction

Crude antigens

Egg lysate

P2J*

Miraci-dium

Cercaria

Schistoso-mulum

Adult worm

Amicon concentrated

2+

3+

>+

+

<+

>+

Ion-exchange purified

2+

2+

+

+

<+

+

Precipitated by 50% (NH)2SO4 solution

3+

2+

>+

+

<+

>+

Immunized mouse serumα

3+

3+

>+

+

<+

>+

Normal mouse serumβ

-

-

-

-

-

-

Antibody-free culture mediumω

-

-

-

-

-

-

  1. *Crude antigens extracted from S. haematobium infected human urine.
  2. α Positive control sample.
  3. β Negative control sample.
  4. ω Background blank sample.
  5. + Positive reaction.
  6. - Negative reaction.
  7. > + Stronger than positive, but weaker than 2+.
  8. <+ Weak reaction or trace.
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BMC Infectious Diseases

ISSN: 1471-2334

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