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Figure 6 | BMC Infectious Diseases

Figure 6

From: Therapeutic and immunomodulatory activities of short-course treatment of murine visceral leishmaniasis with KALSOME™10, a new liposomal amphotericin B

Figure 6

Immunomodulatory role of KALSOME™10 on prophylactic model. Normal healthy mice were treated with different doses of KALSOME™10 (3.5 mg/kg single, 7.5 mg/kg single and double), AmBisome (3.5 mg/kg single, 7.5 mg/kg single and double) and AmB (2.5 mg/kg) in seven different groups with four mice in each group. One group was kept as normal healthy control. Spleen cells of differently treated animals were isolated 10 days after treatment, plated aseptically (2.5 × 105 cells/well), and stimulated with Con A (2.5 μg/ml). Levels of ConA-specific IFNγ (A) and IL-10 (B) were determined after 72 hours of incubation by ELISA. Mean frequencies of CD4+IFNγ+ cells per total CD4+ cells (C), CD8+IFNγ+ cells per total CD8+ cells, (D) CD4+IL-10+ cells per total CD4+ cells (E) and CD8+IL-10+ cells per total CD8+ cells (F) of ConA stimulated splenocytes were determined by flow cytometry after 12 hours of incubation. Data represented as means ± SE for four mice per group and are representative of two independent experiments with similar results. *P < 0.05, **P < 0.01, ***P < 0.001.

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