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Table 1 Assessment of different parasitological parameters and protective efficacy in pre-immunized mice challenged with S. mansoni cercariae

From: Fusion protein comprised of the two schistosomal antigens, Sm14 and Sm29, provides significant protection against Schistosoma mansoni in murine infection model

Animal group

Adult worm burden ± SE

%R

Eggs/gram liver ± SE

%R

Eggs/gram intestine ± SE

%R

Mean no. of granulomas/LPF ± SE

%R

Mean granuloma diameter (μm) ± SE

%R

Negative control

22.714 ± 2.6

-

2489.857 ± 172

-

3803.571 ± 352.4

-

10.714 ± 0.5

-

285.857 ± 12.1

-

Poly (I:C)

21.778 ± 2.4

4.1NS

1918.556 ± 220

22.9NS

3586.444 ± 181.6

5.7NS

8.444 ± 0.5

21.2†

254.444 ± 10.1

11NS

Sm14

22.1 ± 1.9

2.7NS

2452.2 ± 167.2

1.5NS

3806.1 ± 195.5

0.1NS

7.8 ± 0.29

27.2**

216.778 ± 6.8

25**

Sm14+ poly (I:C)

19.429 ± 3.1

14.5NS

1878.571 ± 104

24.6NS

2702 ± 158.6

28.96†

8 ± 0.58

25.33*

227.857 ± 11.3

20.3*

Sm29

18.625 ± 2

18NS

1489.5 ± 97.1

40.2*

2604.875 ± 132.1

31.5*

7.375 ± 0.46

31.2**

219.375 ± 7.9

23.3**

Sm29+ poly (I:C)

21.222 ± 1.5

6.6NS

2042.111 ± 230

18NS

3502.889 ± 219

7.91NS

7.556 ± 0.5

29.5**

240 ± 10.1

16†

FSm14/29

12.556 ± 1.2

44.7†

659.222 ± 116.5

73.5**

888.667 ± 89.3

76.64**

4.1 ± 0.23

61.7**

152.556 ± 7.7

46.6**

FSm14/29 + poly(I:C)

11.714 ± 1.7

48.4†

428.857 ± 103.6

82.8**

1033.857 ± 128.4

72.82**

3 ± 0.31

72**

102.571 ± 6.4

64.1**

  1. (NS): statistically non-significant; (†): p value < 0.05; (*): p value < 0.01; (**): p value < 0.001 when compared to the negative control group using the ANOVA test with post-hoc Tukey-Kramer test. SE: standard error of the mean. LPF: low power field. %R: percentage reduction as compared to the negative control. All results shown for groups immunized with adjuvanted or unadjuvanted FSm14/29 were significantly different (p < 0.05) from all other groups including the negative control, the adjuvant and the individual antigens groups using the ANOVA test with post-hoc Tukey-Kramer test.