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Table 2 Agreement with possible evaluation criteria of PT for NGS

From: Proficiency testing for bacterial whole genome sequencing: an end-user survey of current capabilities, requirements and priorities

Statement

Strong disagreementStrong agreement

% agreement

17.1 Assessment of the quality of WGS reads is a very important consideration

0

2

4

15

20

85.4%

17.2 Ability to integrate and accommodate sequence data from multiple vendor platforms is a very important consideration

0

2

4

17

18

85.4%

17.3 Capacity for de novo sequencing and genome assembly is a very important consideration

0

3

6

21

11

78%

17.4 Capacity for analysis of emerging biothreats is a very important consideration

0

0

11

19

11

73.2%

17.5 Accurate classification of existing frequently tested and globally relevant pathogens (e.g., foodborne Salmonella) is a very important consideration

0

0

3

22

16

92.7%

17.6 Quality of reference based assembly is a very important consideration

0

3

6

20

12

78%

17.7 Quality of annotation is a very important consideration

0

2

8

24

7

75.6%

17.8 Single nucleotide polymorphism (SNP) calls is a very important consideration

0

2

4

15

20

85.4%

17.9 Tree building is a very important consideration

0

0

4

24

13

90.2%