Colonisation with ESBL-producing and carbapenemase-producing Enterobacteriaceae, vancomycin-resistant enterococci, and meticillin-resistant Staphylococcus aureus in a long-term care facility over one year

Table 3 Correlation of pulsed-field gel electrophoresis (PFGE) clusters with bla type, sequence type, and subclone among 124 antimicrobial-resistant Escherichia coli and Klebsiella pneumoniae isolates from long-term care facility residents

Organism	PFGE cluster	No. of isolates	blagenes, no. of isolates				ST^aand subclone (no. of isolates)
			bla _{CTX-M-group-1} ^b	bla _TEM	bla _SHV	bla _OXA-1
E. coli	EcA^c	91	91	0	0	79	ST131 H30-Rx(91)
E. coli	EcA1^d	2	1	0	0	0	ST131 H30-Rx (1)
E. coli	EcB^e	6	6	0	0	6	ST131 H30-Rx (6)
K. pneumoniae	KpA^f	6	6	0	6	6	ST1236/ST48 (6)^g

^aST = sequence type (from multilocus sequence typing). Inferred from PCR-based assays for E. coli and by PFGE similarity to reference strains of known sequence type (ST) for K. pneumoniae strains.
^bAlthough other bla_CTX-M genes were tested for including bla_{CTX-M-group-2}, bla_{CTX-M-group-8}, bla_{CTX-M-group-9}, and bla_{CTX-M-group-25}, none were detected other than bla_{CTX-M-group-1}.
^cEcA = ESBL-EC (n = 91) represented in PFGE Cluster A and similar to representative of UK Strain A and international PFGE812.
^dEcA1 = ESBL-EC (n = 1) demonstrating 83% similarity to strains in PFGE Cluster A.
^eEcB = ESBL-EC (n = 6) represented in PFGE Cluster B.
^fKpA = ESBL-KP PFGE cluster similar to ST1236.
^gST1236 is a single-locus variant of ST48; both are part of clonal complex 43.

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