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Table 3 Correlation of pulsed-field gel electrophoresis (PFGE) clusters with bla type, sequence type, and subclone among 124 antimicrobial-resistant Escherichia coli and Klebsiella pneumoniae isolates from long-term care facility residents

From: Colonisation with ESBL-producing and carbapenemase-producing Enterobacteriaceae, vancomycin-resistant enterococci, and meticillin-resistant Staphylococcus aureus in a long-term care facility over one year

Organism PFGE cluster No. of isolates blagenes, no. of isolates STaand subclone (no. of isolates)
    bla CTX-M-group-1 b bla TEM bla SHV bla OXA-1  
E. coli EcAc 91 91 0 0 79 ST131 H30-Rx(91)
E. coli EcA1d 2 1 0 0 0 ST131 H30-Rx (1)
E. coli EcBe 6 6 0 0 6 ST131 H30-Rx (6)
K. pneumoniae KpAf 6 6 0 6 6 ST1236/ST48 (6)g
  1. aST = sequence type (from multilocus sequence typing). Inferred from PCR-based assays for E. coli and by PFGE similarity to reference strains of known sequence type (ST) for K. pneumoniae strains.
  2. bAlthough other blaCTX-M genes were tested for including blaCTX-M-group-2, blaCTX-M-group-8, blaCTX-M-group-9, and blaCTX-M-group-25, none were detected other than blaCTX-M-group-1.
  3. cEcA = ESBL-EC (n = 91) represented in PFGE Cluster A and similar to representative of UK Strain A and international PFGE812.
  4. dEcA1 = ESBL-EC (n = 1) demonstrating 83% similarity to strains in PFGE Cluster A.
  5. eEcB = ESBL-EC (n = 6) represented in PFGE Cluster B.
  6. fKpA = ESBL-KP PFGE cluster similar to ST1236.
  7. gST1236 is a single-locus variant of ST48; both are part of clonal complex 43.