Dissemination of VIM-2 producing Pseudomonas aeruginosa ST233 at tertiary care hospitals in Egypt

Table 1 List of primers used in this study

Primers	Sequence 5′-3′	Use	Expected PCR product	Reference
PA-SS-F	GGGGGATCTTCGGACCTCA	Identification	956 bp	Spilker et al. [9]
PA-SS-R	TCCTTAGAGTGCCCACCCG
*bla* _IMP-1	TGAGCAAGTTATCTGTATTC	bla _IMP-1	740 bp	Yan et al. [11]
	TTAGTTGCTTGGTTTTGATG	amplification
VIM-F1	ATGTTCAAACTTTTGAGTAAGTTATT	bla _VIM-2	801 bp	Frasson et al. [12]
VIM-FK	ATGTTAAAAGTTATTAGTAGTTTATTGKTC	amplification and sequencing
VIMR1	CTACTCGGCGACTGAGC
VIMR2	CTACTCAACGACTGAGCGA
*bla* _NDM		bla _NDM	984 bp	Kaase et al. [13]
_PreNDM-A	CACCTCATGTTTGAATTCGCC	amplification
_Pre-NDM-B	CTCTGTCACATCGAAATCGC
*bla* _GIM	TCGACACACCTT GGT CTG AA	bla _GIM	477 bp	Ellington et al. [12]
*bla* _GIM	AACTTCCAACTT TGCCATGC	amplification	477 bp	Ellington et al. [12]
*bla* _SPM	AAAATCTGGGTACGCAAA CG	bla _SPM	271 bp	Ellington et al. [12]
*bla* _SPM	ACATTATCCGCTGGAACAGG	amplification	271 bp	Ellington et al. [12]
*bla* _SIM	TAC AAG GGATTCGGCATCG	bla _SIM	570 bp	Ellington et al. [12]
*bla* _SIM	TAATGG CCTGT CCCATG TG	amplification	570 bp	Ellington et al. [12]
*intI1*		intI1	457 bp	Shibata et al. [15]
Int-1-F	GCA TCC TCG GTT TTC TGG	amplification and sequencing
Int-1-R	GGT GTG GCG GGC TTC GTG	amplification and sequencing