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Table 2 Sampling, isolation and antimicrobial test methods

From: A meta-analysis of the proportion of animal Salmonella isolates resistant to drugs used against human salmonellosis in Ethiopia

Author

Sampling method

Number of animals

Sample matrices

Number of samples

Method of isolationb

Number of drugs

Drug testc

[17]a

all

323

f, m, ad

1292

ISO

17

mic

[18]a

all

119

f, m, ad, l, s

714

ISO

17

mic

[19]

rs

204

f, m, ad, l, s

1224

ISO

24

mic

[12]

rs

101

c, m, ad, l, t

501

ISO

24

mic

[13]a

all

278

c, m, cs

833

ISO

24

mic

[20]

rs

100

r, c, m, cs, h, hs

788

ISO

24

mic

[21]

rs

186

i, m, l, cs

744

ISO

8

dzi

[22]

rs

195

f, ml

390

ISO

10

dzi

  1. ad = abdominal and diaphragmatic muscles; cs = carcass swab; rs = random sampling; c = caecal contents; dzi = diameter of zone of inhibition; f = feces; h = hide; hs = hand swabs at flaying and evisceration; i = intestinal contents; l = liver; m = mesenteric lymph nodes; mic = minimum inhibitory concentration; ml = milk; s = spleen; r = rumen contents; t = tongue.
  2. aAll animals slaughtered on each sampling day were sampled.
  3. bThe bacterial isolation and identification methods were according the International Organization for Standardization (ISO-6579, 1998–2002) [12,13,17-22] and Quinn et al. (Clinical Veterinary Microbiology, printed from1994-2004) [12,13,17-21], and GSS (Global Salmonella Surveillance) and NHS (National Health Service for Wales) [22].
  4. cThe interpretative standards were according to the National Committee for Clinical Laboratory Standards (NCCLS, 1990–2005) [12,13,17-19,21,22] and Clinical Laboratory Standards Institute (CLSI, 2005) [20]; the susceptibility break point levels for ciprofloxacin resistance were < = 0.125 μg/ml [12,13]; < = 0.5 μg/ml [18] and < = 1 μg/ml [17] but not reported in three studies[19,20,22], and the resistance break point levels were > = 1 μg/ml [18] and > = 4 μg/ml [17] but not tested [12,13] and not reported in others [19,20,22].