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Table 2 Optimal DPO primer mix concentrations

From: A multiplex PCR assay for the detection of five influenza viruses using a dual priming oligonucleotide system

Component

H1N1 pmd09

Seasonal H1N1

H3N2

H5N1

Influenza B

Forward Primer (μM)

20

20

20

20

40

Reverse Primer (μM)

20

20

20

20

40

25× primer mix (μM)

0.8

0.8

0.8

0.8

1.6