Figure 4

Detection specificity of sH1N1, H1N1pdm09, H3N2, H5N1 and FluB by multiplex DPO PCR. A: Each of the five primer pairs was applied individually to amplify a pooled mixture of the five different target cDNAs (Lanes 1–5: sH1N1, H1N1pdm09, H3N2, H5N1 and FluB, respectively). B: A pooled mixture of the five primer pairs was applied to each of the five target cDNAs individually for amplification (Lanes 1–6: sH1N1, H1N1pdm09, H5N1, FluB, H3N2 and negative control). C: Detection of other respiratory viruses (Lanes 1–12: positive control, PIV1, PIV2, PIV4, HRV, hMPV, ADV, CoV229E, RSVA, RSVB, BoV and negative control).