Figure 2
Optimized annealing temperature for sH1N1, H1N1pdm09, H3N2, H5N1 and FluB by multiplex DPO PCR. (A: Tm = 46°C; B: Tm = 50°C; C: Tm = 55°C; D: Tm = 60°C). Lanes: M: DNA Marker; 1–6: 107–102 viral particles/μl. 537 bp for H3N2, 401 bp for FluB, 328 bp for H5N1, 231 bp for H1N1pdm09, and 193 bp for sH1N1.