Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010

Table 1 Sequences of the oligonucleotides used for cDNA synthesis, PCR amplification and sequencing reactions

Primer	Sequence 5′-3′	Specificity	Reaction(s)	Nested-PCR amplicon size (bp)
AH1A	CAG ATG CAG ACA CAA TAT GT	H1	RT, 1st-round PCR
AH1FII	AAA CCG GCA ATG GCT CCA AA	H1	RT, 1st-round PCR
AH1B	ATA GGC TAC CAT GCG AAC AA		2nd-round PCR
AH1EII	CTT AGT CCT GTA ACC ATC CT		2nd-round PCR	944
AH3A	CAG ATT GAA GTG ACT AAT GC	H3	RT, 1st-round PCR
AH3DII	GTT TCT CTG GTA CAT TCC GC	H3	RT, 1st-round PCR
AH3B	AGC AAA GCT TTC AGC AAC TG		2nd-round PCR
AH3CII	GCT TCC ATT TGG AGT GAT GC		2nd-round PCR	591
BHAA	GTG ACT GGT GTG ATA CCA CT	B	RT, 1st-round PCR
BHADII	TGT TTT CAC CCA TAT TGG GC	B	RT, 1st-round PCR
BHAB	CAT TTT GCA AAT CTC AAA GC		2nd-round PCR
BHACII	TGG AGG CAA TCT GCT TCA CC		2nd-round PCR	767
AN1/418 F	TTC TGA CCC AAG GTG CTC TA	N1	RT, 1st- and 2nd-round PCR
AN1/EII	TAC TTG TCA ATG GTG AAC GG	N1	1st-round PCR
AN1/1219R	GAA ACT TCC GCT GTA CCC TG	N2	2nd-round PCR	821
AN2A/Multi	AAC ATT ACT GGA TTT GCA CC	N2	RT, 1st-round PCR
AN2DII/1082	CAA AGG CCC AGC CTT TCA CT		1st- and 2nd-round PCR
AN2B	GGT GAC GAG AGA ACC TTA TG		2nd-round PCR	719
H3.938.R	TGT CAG AGG TTT TCA CCG TCG CTT CCA TTT GGA GTG ATG C	HA1	890-909
Others		HA1
H3.101.F	CAG CAC GGC AAC GCT G	NA	101-116
3GSTRV	TGT CAG AGG TTT TCA CCG TC	NA