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Table 2 Cytokine production by splenocytes of immunized mice after stimulation by ROP18 protein at 8-weeks post immunization *

From: Protective efficacy of recombinant canine adenovirus type-2 expressing TgROP18 (CAV-2-ROP18) against acute and chronic Toxoplasma gondii infection in mice

Group (n = 3)

Cytokine production (pg/mL)

Proliferation (Stimulation Index)#

IFN-γ

IL-2

IL-4

IL-10

ROP18

ConA

CAV-2-ROP18 (I)

914.26 ± 36.56a

431.07 ± 28.94a

197.29 ± 29.98a

44.37 ± 38.54a

4.87 ± 0.65a

2.93 ± 0.43a

CAV-2 (II)

44.28 ± 9.37b

47.23 ± 7.59b

52.65 ± 9.24b

38.49 ± 5.48a

0.23 ± 0.06b

3.07 ± 0.19a

PBS (III)

46.13 ± 10.27b

52.60 ± 11.97b

54.87 ± 9.45b

41.36 ± 5.23a

0.26 ± 0.10b

2.81 ± 0.32a

Blank Control (IV)

42.98 ± 6.76b

51.45 ± 3.88b

53.38 ± 5.97b

40.63 ± 6.74a

0.27 ± 0.07b

2.84 ± 0.26a

  1. *Data are reported as mean ± SD.
  2. #Splenocytes from mice were collected 8-weeks after the immunization.
  3. Values for IL-10 and IFN-γ are for 72 h, values for IL-2 and IL-4 are for 24 h.
  4. Within each column, groups with different superscripts are different (P < 0.05).