Figure 1
Schematic representation of the construction of recombinant plasmid pPolyII-CAV-â–³E3-ROP18 by in vitro ligation. E3, the E3 region of CAV-2; CMV, human cytomegalovirus (hCMV) immediate-early gene promoter; polyA, the SV40 early mRNA polyadenylation signal. Bold letters were those enzymes used in plasmid construction.