Ex vivoanalysis of total CD8+T-lymphocyte frequency and apoptosis in human cutaneous leishmaniasis. (A) Total CD8+ T lymphocytes; (B) Effector CD8+ T lymphocytes; (C) Apoptotic-total CD8+ T lymphocytes; (D) Apoptotic-effector CD8+ T lymphocytes. HS - healthy subjects (n = 18); PBT – patients before treatment (n = 8); PDT - patients during treatment (n = 14); PAT - patients after treatment (n = 11). Statistical analyses were performed by Mann Whitney Test. The bars represent the mean ± standard error. Results were considered significant with P < 0.05 - *(P < 0.05) **(P < 0.01) ***(P < 0.001).