Escherichia colistrains of phylogenetic group B2 and D and bacteriocin production are associated with advanced colorectal neoplasia

Kohoutova, Darina; Smajs, David; Moravkova, Paula; Cyrany, Jiri; Moravkova, Monika; Forstlova, Miroslava; Cihak, Michal; Rejchrt, Stanislav; Bures, Jan

doi:10.1186/s12879-014-0733-7

Table 1 Primers used for the detection of colicin genes

From: Escherichia colistrains of phylogenetic group B2 and D and bacteriocin production are associated with advanced colorectal neoplasia

Colicin	Primer	Sequention of the primer	Lenght of the PCR product
A	ColA-F	cgtggggaaaagtcatcatc	475
A	ColA-R	gctttgctctttcctgatgc	475
B	colicinB-F	aagaaaatgacgagaagacg	492
B	colicinB-R	gaaagaccaaaggctataagg	492
D	ColD-F	ctggactgctgctggtgata	420
D	ColD-R	gaaggtgcgcctactactgc	420
E1	colicinE1-F	tgtggcatcgggcgagaata	649
E1	colicinE1-R	ctgcttcctgaaaagcctttt	649
E1-1	cea2F	ggtggaactggaggtagcaa	357
E1-1	ceaR	acgtcgttgttctgcttcct	357
E2	ColE2-F	tgatgctgctgcaaaagag	409
E2	ColE2-R	ttcaaagcgttccctaccac	409
E3	ColE3-F	taagcaggctgcatttgatg	413
E3	ColE3-R	tcggatctggacctttcaac	413
E4	ColE4-F	gaaggctgcatttgatgct	409
E4	ColE4-R	cggatccggacctttaattt	409
E5	ColE3-F	taagcaggctgcatttgatg	430
E5	ColE5-R	ttgaattctcgaatcgtcca	430
E6	ColE6-F	accgaacgtccaggtgtt	399
E6	ColE6-R	tttagcctgtcgctcctgat	399
E7	ColE7-F	gcattctgccatctgaaat	431
E7	ColE7-R	cttctgcccactttctttcg	431
E8	ColE3-F	taagcaggctgcatttgatg	449
E8	ColE8-R	gactgattggcttgtcgtga	449
E9	ColE3-F	taagcaggctgcatttgatg	418
E9	ColE9-R	gacttttctccctccgacct	418
Ia	ColIa-F	gcatgcaaatgacgctctta	473
Ia	ColIa-R	gaggacgccagttctctgtc	473
Ib	ColIb-F	aacgagtgggtcgatgattc	464
Ib	ColIb-R	ccttttctgcgctcgtattc	464
Js	ColJs-F	tcaaaatgtttgggctcctc	254
Js	ColJs-R	taatctgccctgtcccactg	254
K	ColK-F	cagaggtcgctgaacatgaa	469
K	ColK-R	tccgctaaatcctgagcaat	469
L	Col28b(L)-F	tgcatattgaaagcgtcagc	449
L	Col28b(L)-R	caggttatcccctctcacca	449
M	ColM-F	gcttaccacttcgcaaaacc	429
M	ColM-R	gagcgactctccgataatgc	429
N	ColN-F	agcttggcgagtatcttgga	401
N	ColN-R	caacacagccccgaataaac	401
S4	ColS4-F	tatatggcccaactgctggt	456
S4	ColS4-R	cgtaaggacggacacctgtt	456
U	ColU-F	tgattgctgcgagaaaaatg	485
U	ColU-R	tctgacagcctctccctgtt	485
Colicin	Primer	Sequention of the primer	Lenght of the PCR product
Y	ColY-F	gcaggcagaaaagaacaagg	477
Y	ColY-R	cggacgttatttgccttcat	477
5	Col5-F	cattggcaaaagcgaaatct	443
5	Col5-R	tgcaactctggaaacaatcg	443
10	Col10-F	ggttaccggatttcctggat	448
10	Col10-R	ttctagatgcttggcccact	448
Fy	ColFy-Fa	aaattaagcggtgccattgac	580
Fy	ColFy-Fa	ttctaattgcgccagacctt	580

Back to article page