Brain damage in control and BB-94-treated mice after meningococcal challenge. Mice were infected and treated as described in Figure 1. Brains from perfused animals were collected. A-C. Brain hemispheres were cut in 30 μm cryosections and photographed to determine the number of haemorrhagic spots and the areas of bleeding. Macroscopical assessment of cerebral haemorrhages in animals injected with BB-94 or vehicle (A). Enumeration of bleeding spots (B) and measurement of haemorrhagic areas (C) were carried out on 5 comparable brain sections/mouse. D. Following bleeding assessment, brain hemispheres were homogenised, and supernatants were subjected to mouse albumin ELISA to quantify serum albumin in the brain as a marker of BBB breakdown. Data are displayed as ng of albumin per μg of total protein content of the brain. Data are from 3 independent experiments. *p < 0.05; **p < 0.01.