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Table 2 Cytokine production by splenocyte a cultures from immunized BALB/c mice

From: Alpha-galactosylceramide enhances protective immunity induced by DNA vaccine of the SAG5D gene of Toxoplasma gondii

Group Cytokine production (pg/mL) b
IFN-γ IL-4 IL-10
PBS 52.07 ± 8.23 36.36 ± 5.25 36 ± 4.98
pEGFP-C1 53.15 ± 8.71 39.24 ± 6.43 37.4 ± 6.33
α-GalCer 343.6 ± 50.88* 75.46 ± 5.7* 37.56 ± 3.66
pEGFP-C1-SAG5D 573.43 ± 60.2* 36.65 ± 3.52 40.77 ± 5.66
α-GalCer/pEGFP-C1-SAG5D 781.36 ± 57.57*# 76.73 ± 6.08* 41.33 ± 5.72
  1. aSplenocytes from 4 mice per group two weeks after the final immunization. bValues for IFN-γ at 96 hr, IL-4 at 24 hr, IL-10 at 72 hr are expressed as mean ± SD. *Compared with PBS or pEGFP-C1 group, P < 0.05; #compared with pEGFP-C1-SAG5D or α-GalCer, P < 0.05.