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Table 1 The immunization process of BALB/c mice

From: Alpha-galactosylceramide enhances protective immunity induced by DNA vaccine of the SAG5D gene of Toxoplasma gondii

Group

Immunization time a

First time

Second time

Third time

PBS

100 μl

100 μl

100 μl

pEGFP-C1

100 μg

100 μg

100 μg

α-GalCer

100 μl PBS

100 μl PBS

2 μg α-GalCerb

pEGFP-C1-SAG5D

100 μg

100 μg

100 μg

α-GalCer/pEGFP-C1-SAG5D

100 μg

100 μg

100 μg

  

pEGFP-C1-SAG5D

pEGFP-C1-SAG5D/2 μg α-GalCer

  1. aAll mice were injected three times at two week interval.
  2. b2 μg α-GalCer was diluted with 100 μl PBS before use.