Figure 1From: Alpha-galactosylceramide enhances protective immunity induced by DNA vaccine of the SAG5D gene of Toxoplasma gondii Identifications of SAG5D gene. (A) Constructed plasmid maps and identification of the recombinant plasmids with restriction enzyme digestion. DNA Mark (lane M), pEGFP-C1 digested with BamH I (lane 1), pEGFP-C1-SAG5D digested with BamH I (lane 2), pEGFP-C1-SAG5D digested with Kpn I and BamH I (lane 3). (B) In vivo expression analysis of the constructs in mice by RT-PCR. DNA Mark (lane M), PBS (lane 1), α-GalCer (lane 2), pEGFP-C1 (lane 3), pEGFP-C1-SAG5D (lane 4), pEGFP-C1-SAG5D/α-GalCer (lane 5).Back to article page