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Table 2 Molecular analysis of three confirmed cases infected with avian influenza A (H7N9) virus, Zhejiang Province, China

From: A family cluster of three confirmed cases infected with avian influenza A (H7N9) virus in Zhejiang Province of China

Protein *

Mutations responsible for phenotype change

Position

Family cluster isolates

The human spondaic isolates

H7N9 vaccine candidate isolate

The animal isolates

Index case

Case 2

Case 3

A/Zhejiang/1/2013

A/Zhejiang/22/2013

A/Zhejiang/01/2014

A/Shanghai/1/2013

A/Anhui/1/2013

A/chicken/Jiangsu/2013

HA

Cleavage site

 

PEIPKGR*G

PEIPKGR*G

PEIPKGR*G

PEIPKGR*G

PEIPKGR*G

PEIPKGR*G

PEIPKGR*G

PEIPKGR*G

PEIPKGR*G

RBS positions (H3 numbering)

          

Q226L

226

L

L

L

I

L

L

Q

L

L

G228S

228

G

G

G

G

G

G

G

G

G

Glycosylation motifs

 

30NGTK, 46NATE, 249NDTV, 421NWTR, 493NNTY

30NGTK, 46NATE, 249NDTV, 421NWTR, 493NNTY

30NGTK, 46NATE, 249NDTV, 421NWTR, 493NNTY

30NGTK, 46NATE, 249NDTV, 421NWTR, 493NNTY

30NGTK, 46NATE, 249NDTV, 421NWTR, 493NNTY

30NGTK, 46NATE, 249NDTV, 421NWTR, 493NNTY

30NGTK, 46NATE, 249NDTV, 421NWTR, 493NNTY

30NGTK,46NATE, 249NDTV, 421NWTR, 493NNTY

30NGTK, 46NATE, 249NDTV, 421NWTR, 493NNTY

NA

Stalk

 

69-73 Del

69-73 Del

69-73 Del

69-73 Del

69-73 Del

69-73 Del

69-73 Del

69-73 Del

69-73 Del

Antiviral resistance (Oseltamivir)

          

E120V

120

E

E

E

E

E

E

E

E

E

 

H276Y

276

H

H

H

H

H

H

H

H

H

 

R294K

294

R

R

R

R

R

R

K

R

R

PB2

Enhanced polymerase activity and increased virulence in mice

          

L89V

89

V

V

V

V

V

V

V

V

V

Q591K

591

Q

Q

Q

Q

Q

Q

Q

Q

Q

E627K

627

E

E

E

E

E

K

K

K

E

D701N

701

D

D

D

N

D

D

D

D

D

PB1

H5 virus transmissible among ferrets

          

H99Y

99

H

H

H

H

H

H

H

H

H

I368V

368

V

V

V

I

V

V

I

V

V

PB1-F2

Full length

 

90 aa

90 aa

90 aa

90 aa

90 aa

90 aa

90 aa

90 aa

99a

M1

Increased virulence in mice

          

N30D

30

D

D

D

D

D

D

D

D

D

T215A

215

A

A

A

A

A

A

A

A

A

M2

Antiviral resistance (amantadine)

          
 

S31N

31

N

N

N

N

N

N

N

N

N

NS1

Increased virulence in mice

          

P42S

42

S

S

S

S

S

S

S

S

S

V192I

192

V

I

I

V

V

V

V

V

V

Decreased virulence in mice

218

Del

Del

Del

Del

Del

Del

Del

Del

Del

NP

A22T

22

T

T

A

T

A

A

A

A

A

R246 K

246

K

K

K

R

R

R

R

R

R

 

V280A

280

V

A

A

V

V

V

V

V

V

 

N321S

321

N

N

N

S

N

N

N

N

N

 

D375E

375

D

D

D

D

D

D

E

D

D

PA

I308V

308

I

I

I

V

I

I

I

I

I

 

T618K

618

T

T

T

T

T

T

T

T

T

  1. Single letters refer to the amino acid (Aa) found in the noted protein at a specific site. *The numbering starts with the first condon of Methionine for these proteins.
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BMC Infectious Diseases

ISSN: 1471-2334

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