Performances of two different panfungal PCRs to detect mould DNA in formalin-fixed paraffin-embedded tissue: what are the limiting factors?

Table 4 Results of external quality control panel for detection of Aspergillus DNA from QCMD 2010

Sample participants* No	Sample content	Sample matrix	Sample concentration	Fungal PCR		% correct results
Sample participants* No	Sample content	Sample matrix	Sample concentration	ITS1-2	ITS2	% correct results
01	A. fumigatus DNA	Plasma	30,000 G.eq/ml	+	+	88.9
04	A. fumigatus DNA	Plasma	3000 G.eq/ml	+	+	70.4
02	A. fumigatus DNA	Plasma	300 G.eq/ml	-	-	25.9
03	Clinical negative	Plasma		-	-	81.5
05	A. fumigatus DNA	TE buffer	30,000 G.eq/ml	+	+	88.9
07	A. fumigatus DNA	TE buffer	3000 G.eq/ml	-	-	48.1
09	A. fumigatus DNA	TE buffer	300 G.eq/ml	-	-	29.6
06	Analytical negative	TE buffer		-	-	77.8
13	A. fumigatus conidia	Plasma	1000 Con/ml	+	+	77.8
10	A. fumigatus conidia	Plasma	100 Con/ml	-	-	59.3
11	A. fumigatus conidia	Plasma	10 Con/ml	-	-	40.7
12	Clinical negative	Plasma		-	-	85.2

*n = 27, with permission of QCMD, Glasgow, Scotland; G.eq, Genome equivalent; Con, Conidia.
+, positive, A. fumigatus; −, negative.

ISSN: 1471-2334