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Table 3 Overview of storage period, ß-globin PCR of FFPE DNA, and ITS-PCR-based fungal result

From: Performances of two different panfungal PCRs to detect mould DNA in formalin-fixed paraffin-embedded tissue: what are the limiting factors?

Sample no

Storage in years

Spiking

ß-globin PCR [amplicon length,bp]

ITS-PCR-based identification (sequence length of ITS2 / ITS1-2 in bp)

2 μl

0.5 μl

1

7

+

(+)

(+) [262]

H. aspergillata (376 / 541)

15

7

-

-

-

-

2

6

-

-

-

A. fumigatus (355 / negative)

9

6

+

(+)

(+) [262]

A. flavus (349 / 528)

7

5

-

-

-

A. fumigatus (354 / negative)

19

5

-

-

-

-

14

5

-

-

-

-

17

4

-

-

-

-

11

4

-

-

(+) [262]

A. nidulans (355 / 416)

3

4

+

-

-

probably A. lentulus (349 / 115 partial)

4

4

+

-

(+) [262]

A. fumigatus (354 / 567)

5

4

+

-

(+) [262]

A. fumigatus (353 / negative)

20

4

+

-

(+) [262]

-

21

3

+

-

(+) [262]

-

16

3

+

+

+ [408]

-

6

3

+

+

+ [408]

H. aspergillata (383 / 641)

12

2

+

(+)

(+) [262]

-

18

2

+

+

+ [408]

A. fumigatus (350 / 177 partial)

22

2

+

+

+ [408]

-

13

2

-

(+)

(+) [262]

-

10

1

+

+

+ [408]

Alternaria sp. (346 / 585)

26

1

+

+

+ [408]

-

8

1

+

+

+ [408]

Rhizopus microsporus/azygosporus (372 / 628)

23

1

+

+

+ [408]

A. flavus (342 / 408)

25

1

+

+

+ [408]

-

24

1

+

+

+ [408]

H. aspergillata (384 / 554)

27

1

-

-

-

-

  1. Bands after agarose gel electrophoresis: +, strong; (+), faint; −, absent.