Performances of two different panfungal PCRs to detect mould DNA in formalin-fixed paraffin-embedded tissue: what are the limiting factors?

Table 2 PCR amplification from the different volumes of DNA extract of the 14 PCR-positive FFPE specimens

Sample No	ITS1-2				ITS2				PCR-based identification
Sample No	10 μl	5 μl	2 μl	0.5 μl	10 μl	5 μl	2 μl	0.5 μl	PCR-based identification
1	-	-	+	+	-	+	+	+	H. aspergillata
2	-	-	-	-	-	-	+	+	A. fumigatus
3	-	+*	-	-	-	-	+	+	Probably A. lentulus
4	-	-	-	+	-	-	+	+	A. fumigatus
5	-	-	-		-	-	-	+	A. fumigatus
6	-	-	+	+	+	+	-	+	H. aspergillata
7	-	-	-	-	-	-	+	+	A. fumigatus
8	-	-	-	-	-	-	-	+	Rhizopus microsporus/azygosporus
9	+	+	+	+	-	+	+	+	A. flavus
10	+	+	+	+	-	+	+	+	Alternaria spp
11	-	-	+	+	-	-	+	+	A. nidulans
18	-	-	+	-	-	+	+	+	A. fumigatus
23	-	-	+	-	-	+	+	+	A.flavus
24	-	-	+	-	-	+	+	+	H. aspergillata
Total positive PCR signals	2	2	9	6	1	6	11	14

ISSN: 1471-2334