Table 1 Results of culture, histopathology, and PCR from fresh tissue compared to PCR from FFPE specimens (n = 27)
Patient | Tissue site (storage in years) | Culture | Histopathology | ITS1-2PCR on fresh tissue | PCR on FFPE | Identification after sequencing (% identity) | |
|---|---|---|---|---|---|---|---|
ITS1-2 | ITS2 | ||||||
Culture positive samples | |||||||
1 | Lung (7) | Aspergillus nidulans | + | ND | + | + | Hormographiella aspergillata (100) |
2 | Lung (6) | A. fumigatus/Fusarium sp. | - | ND | - | + | A. fumigatus (99) |
3 | Lung (4) | A. fumigatus | + | ND | - | + | probably A. lentulus (100) |
4 | Lung (4) | A. fumigatus | + | ND | + | + | A. fumigatus (100) |
5 | Lung (4) | A. fumigatus | + | ND | - | + | A. fumigatus (99) |
6 | Lung (3) | H. aspergillata † | + | ND | + | + | H. aspergillata (100) |
7 | Lung (5) | A. fumigatus | + | ND | - | + | A. fumigatus (99) |
8 | Lung (1) | Rhizopus sp. | + | ND | + | + | Rhizopus microsporus/azygosporus (99) |
9 | Skin (6) | A. flavus | ND | ND | + | + | A. flavus (100) |
10 | Skin (1) | Alternaria sp | ND | ND | + | + | Alternaria sp. (99) |
11 | Lung (4) | A. nidulans | + | ND | + | + | A. nidulans (100) |
12 | Lung (2) | A. fumigatus | - | ND | - | - | - |
13 | Lung (2) | H. aspergillata † | + | ND | - | - | - |
14 | Lung (5) | A. fumigatus | + | ND | - | - | - |
15 | Soft tissue (7) | Rhizopus sp. | + | ND | - | - | - |
16 | Cerebellum (3) | H. aspergillata*† | + | ND | - | - | - |
17 | Lung (4) | A. flavus | + | ND | - | - | - |
Histopathology-positive, culture-negative samples | |||||||
18 | Small intestine (2) | - | + | ND | + | + | A. fumigatus (100) |
19 | Sinus sphenoidalis (5) | - | + | ND | - | - | - |
20 | Lung (4) | - | + | ND | - | - | - |
21 | Lung (3) | - | + | ND | - | - | - |
22 | Lung (2) | - | + | ND | - | - | - |
PCR positive, culture-negative samples | |||||||
23 | Lung (1) | - | + | A. flavus | + | + | A. flavus (100) |
24 | Lung (1) | - | + | H. aspergillata | + | + | H. apergillata (99) |
25 | Lung (1) | - | - | R. pusillus/tauricus | - | - | - |
26 | Lung (1) | - | + | R. pusillus/tauricus | - | - | - |
27 | Lung (1) | - | + | Lichtheimia corymbifera | - | - | - |