Table 1 Primers for porA , fetA and fHbp PCR reactions

porA 1^st round forward primer

5′-AAA CTT ACC GCC CTC GTA-3′

porA 1^st round reverse primer

5′-TTA GAA TTT GTG GCG CAA ACC GAC-3′

porA 2^nd round forward primer^a

5′-CCG CAC TGC CGC TTG CGG-3′

porA 2^nd round reverse primer^a

5′-CGC ATA TTT AAA GGC ATA-3′

fetA (segment 1) 1^st round forward primer^b

5′-CGG CGC MAG CGT ATT CGG-3′

fetA (segment 1) 1^st round reverse primer^c

5′-GCG GTT TGA TTT CCT GAT GG-3′

fetA (segment 1) 2^nd round forward primer^a

5′-TTR TTC TCT TAC AAC CGC ARC-3′

fetA (segment 1) 2^nd round reverse primer^a,c

5′-GCG GTT TGA TTT CCT GAT GG-3′

fetA (segment 2) 1^st round forward primer

5′-GCC TTG CCG AAC AAA CYC-3′

fetA (segment 2) 1^st round reverse primer

5′-CGC CCA ATT CGT AAC CGT-3′

fet A (segment 2) 2^nd round forward primer^a

5′-TCA ACT ACC GCC ATC AGG A-3′

fetA (segment 2) 2^nd round reverse primer^a

5′-TTT GAT GGT YTG CCA RAA GTA GC-3′

fHbp 1^st round foward primer

5′-GTG AAY CGA ACT RCC TTC TRY-3′

fHbp 1^st and 2^nd round reverse primer^a

5′-TGT TCR ATT TTG CCG TRY-3′

5′-TGG TIT TTT TYA TCC GGC TTR-3′

fHbp 2^nd round forward primer^a

5′-CCC TGA TTC TGA CCG CCT G-3′