Alignment of the primers and probes for the
-based FRET-qPCR and nested PCR with 20
species. Panel A shows the nucleotide sequences of the primers and probes used in the FRET-qPCR and the corresponding sequences of 20 Rickettsia species. Panel B shows the nucleotide sequences of the primers used for the nested PCR. In both Panels, dots indicate that nucleotides are identical to the primers. The nucleotides between oligonucleotides are not shown. The upstream primer and probes were used as shown while the downstream primer was used as an antisense oligonucleotide.