Figure 2From: Antibacterial properties of Acinetobacter baumanniiphage Abp1 endolysin (PlyAB1) Cloning and over-expression of PlyAB1. (A) Cloning of plyAB1 into pET28a: 1) recombinant plasmid pET28a-plyAB1, 2) pET28a-plyAB1 digested with BamHI and Xhol, 3) PCR amplification of plyAB1. (B) The purified PlyAB1 protein after Ni+ affinity chromatography and filtration.Back to article page