Figure 4

The effects of the complexes on HDAC and PKC activity. At a cut-off of ≥50% inhibition as inhibitory, complex 1 and 2 did not appreciably inhibit HDAC. The positive control, TSA, inhibited the enzyme by 103.7% with a p value of <0.0001 (A). The enzyme control is represented as 100% inhibition. In the PKC assay, significant increases in PKC activity of 31.5 (p = 0.033) and 32.7% (p = 0.036) were observed for 1 and 2 respectively compared to the untreated vehicle control (B) suggesting that reactivation was as a result of an effect on NF-κB. Prostratin, a PKC activator caused significant increases in PKC of 80.3% (p = 0.02). The vehicle control sample is represented as 100% activity.