Figure 3

Complex 1 and 2 reactivate HIV replication in latently infected U1 cells. At non toxic concentrations of 0.2 and 0.5 μM viral p24 levels increased by 2.7 and 2.3 fold for complex 1 and 2 respectively (*p ≤ 0.03) while 200 μM HU, which was included in the study as a cytostatic positive control, reactivated latent virus by 2.6 fold (A). PMA (3 nM), which was used both as a positive control for virus activation and to monitor the effect of the complexes on activated virus, significantly activated viral production (p = 0.01). HU also significantly (p = 0.01) inhibited PMA mediated latency activation by 44% when compared to PMA treated cells only, an observation which was absent for 1 and 2 (B). The p24 levels for the vehicle control and PMA treated cells are represented as 100% so that differences resulting from complex effects could easily be differentiated.