Figure 4
S. pneumoniae administration elicited elevations in inflammatory cytokine levels within the CNS (A and B). Infection was induced via direct intracisternal injection of vehicle (0) or S. pneumoniae (1 × 106 bacteria). On day 1 (D1), D2, and D3 postinfection, brain tissue homogenates were isolated for assessment of TNF-a (Panel A) and IL-6 (Panel B) protein expression by specific capture ELISA. The asterisks indicate statistically significant differences compared to the uninfected animals (p < 0.05).