The activation of NF-kB is increased following infection with
(A). Vehicle (0) or S. pneumoniae (1 × 106 bacteria) was administered to the rats via intracisternal injection. At days 1 (D1), D2, and D3 postinfection, the brain tissue was isolated, and the nuclear protein was prepared for immunoblot assays of the presence of NF-kB p65 (Panel A). The bands were quantified by densitometric analyses and normalized to β-actin expression (Panel B). The asterisks indicate statistically significant differences compared to the uninfected animals (p < 0.05).