Table 2 Oligonucleotide sequences of primers used in this study
Name | Sequence (5′→3′) | Nucleotide position | Polarity |
|---|---|---|---|
Primers for rolling circle amplification (RCA) | |||
RCA1 | AATCCTCACAATA*C*C | 226-240 | Sense |
RCA2 | ACCTATTCTCCTC*C*C | 1744-1758 | Antisense |
RCA3 | CCTATGGGAGTGG*G*C | 637-651 | Sense |
RCA4 | CCTTTGTCCAAGG*G*C | 2675-2689 | Antisense |
RCA5 | ATGCAACTTTTTC*A*C | 1814-1828 | Sense |
RCA6 | CTAGCAGAGCTTG*G*T | 15-29 | Antisense |
RCA7 | TAGAAGAAGAACT*C*C | 2368-2382 | Sense |
RCA8 | GGGCCCACATATT*G*T | 2585-2599 | Antisense |
Primers for in situ PCR (IS-PCR) | |||
Pup58 | CCCCGTCTGTGCCTTCTC | 1547- 1564 | Sense |
Pdown84 | CAGCTTGGAGGCTTGAACAGT | 1859-1879 | Antisense |