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Figure 4 | BMC Infectious Diseases

Figure 4

From: A novel method for detection of HBVcccDNA in hepatocytes using rolling circle amplification combined with in situ PCR

Figure 4

IS-PCR and immunohistochemical and HE staining for detecting HBV cccDNA in FFPE liver biopsies of HCC patient. HBVcccDNA were detected in the liver tissues by PSAD + RCA + IS-PCR (c), whereas no positive signal was detected in the tissue sections that were subjected to IS-PCR (a) and PSAD + IS-PCR (b). (d) hematoxylin-eosin staining of FFPE liver biopsies of HCC patient. The arrow in the images shows HBVcccDNA positive signals in the hepatocyte nuclei. Original magnifications ×400.

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