Evidence of West Nile virus infection in Nepal

BMC Infectious Diseases

Table 1 Genetic distance of the WNV sequence fragments from the lineage 1a and 2 reference strains

Specimen ID.	Fragment ID.	Fragment length (bp)	Genetic distance (p - distance)┼
			Lineage 1a		Lineage 2
			EU081844 Egypt 1951	EU249803 India 1968	M12294 Uganda 1937	AY532665 Uganda 1937
VIROAF73	i.	297	0.000	0.000	0.226	0.0234
	ii.	207	0.000	0.000	0.189	0.184
	iii.	400	0.003	0.003	0.229	0.229
	iv.^a	236	0.242	0.242	0.013	0.004
	v.	364	0.000	0.008	0.206	0.206
	vi.	467	0.000	0.000	0.221	0.218
	vii.	270	0.056	0.056	0.211	0.219
	viii.	262	0.011	0.011	0.229	0.229
	ix.	208	0.000	0.000	0.216	0.207
	x.	450	0.007	0.007	0.258	0.258
	xi	281	0.000	0.000	0.224	0.224
	xii.	523	0.048	0.048	0.225	0.225
VIROAF74	i.	270	0.000	0.000	0.233	0.238
	ii.	401	0.083	0.085	0.261	0.261
	iii.	252	0.129	0.133	0.325	0.325
	iv.	492	0.018	0.020	0.226	0.226
	v.	473	0.010	0.010	0.185	0.185
	vi	419	0.005	0.005	0.232	0.234
	vii.	352	0.006	0.006	0.179	0.179
	viii.	221	0.000	0.000	0.213	0.213
	ix.	410	0.002	0.002	0.242	0.24
	x.	376	0.015	0.015	0.249	0.249
	xi.^a	252	0.239	0.239	0.016	0.008
	xii.	942	0.007	0.008	0.203	0.203
	xiii.	345	0.006	0.006	0.109	0.106

┼Closely related sequence to VIROAF73 & VIROAF74 by blastn on GENBANK: Lineage 1a Reference Strains: Egypt 1951 (EU081844) and India 1968 (EU249803) and Lineage 2 Uganda 1937 (M12294 and AY532665).
^aFragments identified to be closely related to Lineage 2.
Numbers in bold indicate the differences of genetic distance from the Egypt 1951 (EU081844) and India 1968 (EU249803).

ISSN: 1471-2334