Figure 1
From: First case of infective endocarditis caused by Helicobacter cinaedi
Conventional 40-cycle Helicobacter cinaedi PCR 1 and 2 targeting the cdt gene resulting in 659-bp and 359-bp amplicons, respectively. Polyacrylamid-based gel electrophoresis of PCR products using Agilent 2200 Tape Station (Agilent Technologies). L, molecular weight ladder; lane 1 and 6, 400 ng DNA of aortic valve (formalin-fixed); lane 2 and 7, 80 ng DNA of aortic valve (formalin-fixed); lane 3 and 8, 250 ng DNA of aortic valve (fresh); lane 4 and 9, 50 ng DNA of aortic valve (fresh); lane 5 and 10, negative control (5 μl of elution buffer).