Figure 2

IS1081-PCR of DNA extracted directly from pleural fluid and lymph node aspirate of lane 1 represents a 100 bp DNA ladder (molecular marker), Lane 2 through lane 4 are DNA extracted from pleural fluid, Lane 5 and lane 6 are DNA extracted samples from lymph node aspirates, Lane 7 thorough 9 represent an amplification product of the positive controls, i.e. M. tuberculosis strain H37Rv DNA, known ZN-positive and culture-positive test sample, and positive sample from previous PCR run which showed positive result, Lane 10 through 12, i.e. an extraction control, a reaction with substitution of Qiagen RNase free-water for the test template, and a negative sample from previous PCR assay gave a negative result.