Evaluation of the presence and zoonotic transmission of Chlamydia suis in a pig slaughterhouse

BMC Infectious Diseases

Table 1 Results of the molecular analyses on contact surface samples at different locations in the abattoir

Location	PCRII	ViableC. suis
Pig reception area	-	-
Slaughtering and bleeding area	+	+
Pre-washing bath location	-	-
Dehairing area	+	+
Cutting/deboning area	-	-
Carcass splitting area	+	-
Organ evisceration area	-	-
Individual weighing area	+	+
Chilling/hanging room	-	-
Pig intestine washing room	+	-
Employee dining room	+	+
Sanitary facilities at the individual wheighing area	+	-
Sanitary facilities at the pig intestine washing room	+	+
Sanitary facilities at the cloakrooms	+	+
Total (positives/examined)	9/14	6/14

Swabs in DNA/RNA stabilization buffer were used to detect the presence of C. suis DNA by use of PCRI and II. The results of the PCRII analysis are mentioned in column `PCRII’. Swabs in 2-SP medium were inoculated on Vero-cells for evaluation of the presence of viable Chlamydiae. Culture positive chlamydial isolates were analysed using PCRII for subsequent molecular detection of C. suis. Results are shown in column `viable C. suis’. All culture positive samples showed isolation score 1.

ISSN: 1471-2334