Table 1 The primers, nucleotide numbers and the thercycling conditions

Eecf-F1

ttg tgt tcc aga tgg cca gc

(49–68)*

95'C

2 min × 1

First round: 307

Eecf-R1

ctt aag gct gcc gtg ttt gc

(356–337)*

95'C

30 sec

Eecf-F2

gaa gca acc aar ttc tgt gc

(115–134)*

60'C (57'C)#

1 min × 44

Eecf-R2

aaa cct atg tcc ttc ctc c

(326–308)*

72'C

2 min

Second round: 211

72'C

10 min × 1

Gre-F1

aat gtg ccg aac ttg gac ag

(170–189)**

95'C

2 min × 1

First round: 593

Gre-R1

tgc gac aag tgc aat ccc g

(751–733)**

95'C

30 sec

Gre-F2

atc aga tgg cca gtg caa cc

(198–217)**

57'C

1 min × 44

Gre-R2

act ccc tgc acc act caa tg

(665–646)**

72'C

2 min

Second round: 469

72'C

10 min × 1