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Table 1 The primers, nucleotide numbers and the thercycling conditions

From: The first clinical case due to AP92 like strain of Crimean-Congo Hemorrhagic Fever virus and a field survey

  Sequence Position Thermalcycling conditions   Amplicon lengths (bp)
Eecf-F1 ttg tgt tcc aga tgg cca gc (49–68)* 95'C 2 min × 1 First round: 307
Eecf-R1 ctt aag gct gcc gtg ttt gc (356–337)* 95'C 30 sec  
Eecf-F2 gaa gca acc aar ttc tgt gc (115–134)* 60'C (57'C)# 1 min × 44  
Eecf-R2 aaa cct atg tcc ttc ctc c (326–308)* 72'C 2 min Second round: 211
    72'C 10 min × 1  
Gre-F1 aat gtg ccg aac ttg gac ag (170–189)** 95'C 2 min × 1 First round: 593
Gre-R1 tgc gac aag tgc aat ccc g (751–733)** 95'C 30 sec  
Gre-F2 atc aga tgg cca gtg caa cc (198–217)** 57'C 1 min × 44  
Gre-R2 act ccc tgc acc act caa tg (665–646)** 72'C 2 min Second round: 469
    72'C 10 min × 1  
  1. * According to the Hodhza strain (AY223475)
  2. ** According to the AP92 strain (U04958)
  3. # In the second round