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Table 3 Survival of organisms embedded in TBF or BBF1 following HLD2: indirect qualitative outgrowth testing compared to quantitative viability3 results

From: Modeling microbial survival in buildup biofilm for complex medical devices

Microorganism:
BFF
Average cfu/peg4 (Log10) Outgrowth5
# Positive/#Tested
Average time to detection (days) for outgrowth
HLD: 2.6% GLUT:    
P. aeruginosa:    
TBF – Day 3 <LD6 3/9 4
TBF – Day 15 3.7 7/9 1
TBF – Day 30 4.8 9/9 1
BBF – Day 3 <LD 4/9 20
BBF – Day 15 <LD 2/9 10
BBF – Day 30 1.9 2/9 1
HLD: 7% AHP:    
P. aeruginosa:    
TBF – Day 3 <LD 0/9 -
TBF – Day 15 1.6 4/9 1
TBF – Day 30 2.5 4/9 1
BBF – Day 3 <LD 0/9 -
BBF – Day 15 <LD 0/9 -
BBF – Day 30 <LD 0/9 -
  1. 1 3-day cyclical drying/HLD in ATS medium
  2. 2 HLD for cyclic BBF pegs and sample pegs of TBF (formed in ATS)
  3. 3 Quantitative viability testing on replicate pegs (without enhanced recovery protocol)
  4. 4 Average of 9 pins
  5. 5 Indirect qualitative outgrowth (enhanced recovery protocol):
  6. TBF or BBF peg placed in 10%FBS-TSB, shake/sonicate/vortex (as per extraction
  7. method), then continue to incubate at 35°C/30d
  8. 6LD: limit of detection is 10 cfu/peg for quantitative viability counts